红花继木的组织培养包括初代培养、继代增殖培养和生根培养。组培技术在红花继木生产上的应用,不仅仅限于组培育苗,还有望通过组培在育种上的应用,解决种间、属间等远缘杂交和杂种胚停止发育的缺陷,使红花继木dezhongzhiziyuanjinyibuchunhuahetigao。
红花继木苗木图片
1、外植体
xuanshengzhangjianzhuang、wubingchonghaidehonghuajimujingjian、yajizhidengzuowaizhiti,qizhongwaizhiticaijizai7~8yuezuihao,wuranlvjiaodi。
2、培养基
honghuajimudezuzhipeiyangbaokuochudaipeiyang、jidaizengzhipeiyangheshenggenpeiyang。shiyanbiaoming,chudaipeiyanghejidaizengzhipeiyangjikecankaoms+ba1.0mg/l+iba1.0mg/l;shenggenpeiyangjikecankao1/2ms+naa1.0~1.5mg/l+0.1%dehuoxingtan。
3、培养条件
honghuajimudepeiyangtiaojianyibanwei:wendu20℃zuoyou,guangzhaoshijian14h/d(meitian14xiaoshi),guangzhaoqiangduwei1500lx(lekesi)。
红花继木球图片
操作步骤
(1)waizhitixiaodu:cailiaocaihuihou,jishiyongxibodepiaobaifenrongyexiqucailiaoshangdewuwu,erhouyongzilaishuifanfuchongxi20fenzhong,zhizhichongxiganjingbeiyong。
(2)jiezhong:zaijiezhongqian,xianjiangjiezhongshineideziwaidengkaiyue20fenzhongjinxingxiaodu,ranhoudakaijiezhongtaishangdegufengjichuishifenzhongzuoyou,jikejinrujiezhongshijinxingjiezhongcaozuo。jiangxiaohaodudewaizhitifangyuchaojinggongzuotaishang,shouxian,zaiwujuntiaojianxiayong70%jiujinghuo0.01%shenggongzaichangwenxiajinpaocailiaoxiaodu10fenzhong,zaiyongwujunshuichongxi3~4ci。ranhouyongxiaodudeniezijiaqucailiaozhiyupeiyangjishangjinxingwujunpeiyang。zhuyijiezhongshisuoyongdeniezixuxianzai70%dejiujingzhongxiaodu,bingzaijiujingdengshangshaoyixiazaiyong,yiqiugenghaoxiaodu,bimianjiezhongwaizhitiganran。
dangyazhangzhi2limizuoyoushi,qiequyaduanjiezhongzaizengzhipeiyangjishangfenhuazengzhi,ranhoujiangjidaizengzhidecongshengyafenbieyizhishenggenpeiyangjizhongyoudaoshenggen,danggenzhangzhi1~2limizhangshi,congpeiyangshizhongquchulianmiao,yibeiyizai。
(1)fanzhisudukuai:congyouliangmuzhushangquxiayigeyahuoyibufenzuzhi,lixiangtiaojianxiayiniannengfanzhijiwandaoshijiwanzhuxindegeti,suoyizupeijishuzuishihedaliangkuaisufanzhimingguihexiyouzhiwu。
(2)tuodukuaifan:zhenjun、xijun、tebieshibingduduizhiwudeweihaiyuelaiyueda,yishoudaorenmendezhongshi,ercaiyongzhiwujingjianjinxingzuzhipeiyang,zeshiyixiangzuiyouxiao、zuikuaijie、zuishiyongdefangfa。
(3)fuzhuyuzhong:zupeijishushidanbeitiyuzhong、yichuanxingzhuangyanjiu、baoliuzhongzhiziyuandeyitiaoyouxiaotujing。
(4)zupeimiaoxingzhuangyizhi:yongzupeijishufanzhidezhizhulaiziyuyigeqinbenzhuti,suoyinenggoubaochihoudaizhizhujuyougaodudeyizhixinghezhengqidu。
(5)bushouqinbenxianzhi:youyuzupeimiaoshicaiyongwuxingfanzhijishupeiyuzhizhu,suoyibushouqinbenbukaihua、bujiezijizajiaopinzhonghoudaiyibianyidengpinzhongtexingdeyingxiang,kefanzhibuyu、zajiaopinzhongjiyibianyipinzhong。
(6)bushoujijiexianzhi:zupeishengchancaiyongrengongguangzhao,shineipeiyang,keyizhounianshengchan。
(7)jieshengtudiheziyuan:youyuzupeishengchankeyizaishineijizhongshengchan,litifencengshengchan,jizhongguanli,nenggouzuidaxiandudijieshengtudi、renlideng,fuhexiandainongyedefazhanfangxiang。
zupeijishuzaihonghuajimushengchanshangdeyingyong,bujinjinxianyuzupeiyumiao,haiyouwangtongguozupeizaiyuzhongshangdeyingyong,jiejuezhongjian、shujiandengyuanyuanzajiaohezazhongpeitingzhifayudequexian,shihonghuajimudezhongzhiziyuanjinyibuchunhuahetigao。
红花继木苗木基地